Iran said it has expanded attacks across the Gulf after further U.S. strikes on Iranian targets, and it claimed that the Strait of Hormuz is closed again.

The renewed exchange came after Iran fired warning shots related to a vessel attempting to transit the strait on an “unapproved route.” The U.S. retaliated with air operations, including a third round of strikes reported as hitting 140 targets during the overnight period. U.S. officials framed the actions as holding Iranian forces accountable for attacks on commercial shipping and degrading Iran’s ability to target civilian vessels in the waterway.

Iran, for its part, said it responded by striking U.S. facilities and that its forces targeted additional states in the region, including the United Arab Emirates and Qatar. Regional authorities reported intercepts and injuries from falling shrapnel tied to missile and drone activity.

Competing claims also continued over whether the strait is actually open. Iran’s Revolutionary Guards said the waterway would remain closed until the end of what it called U.S. “interference,” while U.S. Central Command said ships were continuing to transit and that American forces were positioned to ensure freedom of navigation.

The escalation further clouded the status of an interim U.S.-Iranian arrangement that was meant to reopen the strait and pause the fighting, with both sides trading blame for alleged breaches as attacks broadened in pace and geography.